PLRP-S™ Analytical Columns

Our PLRP-S™ Analytical Columns

The PLRP-S™ stationary phase, featuring a 1000-Å pore size, is our wide‑pore polystyrene/divinylbenzene (PS/DVB) material engineered for high‑resolution reversed‑phase chromatography of large biomolecules.

• 1000‑Å wide‑pore polymeric phase

Optimized for intact proteins, monoclonal antibody subunits, long peptides, and oligonucleotides requiring efficient mass transfer.

• 5‑µm particle size for analytical HPLC

Provides a practical balance between resolution and backpressure, suitable for conventional HPLC systems and elevated‑temperature protein separations.

• Silica‑free PS/DVB chemistry

Eliminates silanol interactions and metal‑surface effects, improving peak shape for highly charged or hydrophobic biomolecules.

• Extreme chemical and thermal stability

Compatible with aggressive cleaning protocols and high‑temperature operation (up to 200 °C).

• Broad pH range (1–14)

Supports method development flexibility and long column lifetimes under harsh conditions.

Our columns are supplied without connectors: within our Deans Column Switching System, the proximal end of a column is mounted directly into FingerTight® 360-µm MicroTees (Part Number P-888, IDEX Health & Science®, Oak Harbor, WA, USA). The distal end is butt-connected to our NanoElectroSpray Tip, preferably using a 1/16-in. OD × 0.012-in. ID PTFE sleeve (e.g., Supelco® Part Number 58702).

The tubing length of each Analytical Column is approximately 5 cm longer than the column bed length. To ensure optimal integration with our Deans Column Switching System, the tubing should be shortened to the proximal end of the stationary phase by cutting the column prior to installation.