Trapping Columns

Use these buttons to quickly access our Trapping Columns

Our portfolio of Solid Phase Extraction Trapping Columns

All our Trapping Columns are designed to accommodate sample injection volumes in the typical range of 1-50 µL, while ensuring optimal mobile phase flow rates of 10-200 nL/min during analytical separation.

These columns are supplied without connectors. Within our Deans Column Switching System, they are mounted directly into FingerTight® 360-µm MicroTees (Part Number P-888, IDEX Health & Science®, Oak Harbor, WA, USA).

The tubing length of each Trapping Column is approximately 10 cm. To ensure optimal integration with our Deans Column Switching System, the tubing should be shortened to 3.6-4 cm by cutting at the proximal end of the column prior to installation.

Available Stationary Phases

Our Trapping Columns are available in a range of stationary phase chemistries:

Single-Phase Trapping Columns are suitable for a wide range of nanoscale LC applications, including complex samples derived from whole-proteome digests. These columns deliver consistent performance with Reversed-Phase Stationary Phases, making them a reliable choice for both routine and high-complexity workflows:
- BioSphere™ C4, particle size=5 µm, pore size=300 Å
- BioSphere™ C8 , particle size=5 µm, pore size=120 Å
- BioSphere™ C18, particle size=5 µm, pore size=120 Å
- PLRP-S™, particle size=5 µm, pore size =1000 Å
  This polystyrene/divinylbenzene (PS/DVB) stationary phase is optimized for intact protein analysis, e.g., mAb subunit analyses
- Porous Graphitic Carbon, particle size=7 µm
- Customer-supplied Ion eXchange stationary phases upon request
Two-Phase Trapping Columns are engineered for challenging samples with high contamination levels. Featuring an upstream Perfusion Phase that shields the downstream Reversed-Phase particles, these columns prevent clogging and significantly extend column lifetime:
- Poros® 10R2, particle size=10 µm, pore size=800 Å
  BioSphere™ C18, particles size=5 µm, pore size=120 Å
Three-Phase Trapping Columns are designed for online Ion eXchange fractionation or PhosphoPeptide enrichment. A specialized stationary phase is embedded between two BioSphere C18 layers, enabling precise and efficient separation or enrichment workflows:
- For online Strong Cation eXchange (SCX) fractionation:
  – BioSphere™ C18, particle size=5 µm, pore size=120 Å
  – PolySULFOETHYL Aspartamide™, particle size=5 µm, pore size=300 Å
  – BioSphere™ C18, particle size=5 µm, pore size=120 Å
- For online PhosphoPeptide (PP) enrichment:
  – BioSphere™ C18, particle size=5 µm, pore size=120 Å
  – Titansphere® TiO₂, particle size=5 µm, pore size=100Å
  – BioSphere™ C18, particle size=5 µm, pore size=120 Å

How to order

Request for Quotation or Order Request can be placed by postal mail or e-mail:

Postal Mail

PO Box 35
3790 CA Achterveld
The Netherlands

E-mail

orders@sothisseparation.com

New to Sothis Separations?

Receive 15% off your first order.^*

50-µm ID Trapping Columns

Recommended for use with our 25-µm ID Analytical Columns
Optimal mobile phase flow rate at trapping stage: 1-2 µL/min

Catalog Number	Column Chemistry	Particle Size	Bed Length	Price (1/pk)	Price (5/pk)
TS04502	C4	5 µm	20 mm	€ 93.-	€ 396.-
TS08502	C8	5 µm	20 mm	€ 93.-	€ 396.-
TS18502	C18	5 µm	20 mm	€ 93.-	€ 396.-
TDR218502	Perfusion C18	10 µm 5 µm	30 mm 10 mm	€ 137.-	€ 583.-
TT18SC182	C18 SCX C18	5 µm 5 µm 5 µm	15 mm 10 mm 20 mm	€ 162.-	€ 689.-
TT18PP182	C18 TiO₂ C18	5 µm 5 µm 5 µm	15 mm 3 mm 20 mm	€ 162.-	€ 689.-

[Top]

75-µm ID Trapping Columns

Recommended for use with our 50-µm ID Analytical Columns
Optimal mobile phase flow rate at trapping stage: 1-3 µL/min

Catalog Number	Column Chemistry	Particle Size	Bed Length	Price (1/pk)	Price (5/pk)
TS04503	C4	5 µm	20 mm	€ 93.-	€ 396.-
TS08503	C8	5 µm	20 mm	€ 93.-	€ 396.-
TS18503	C18	5 µm	20 mm	€ 93.-	€ 396.-
TSPR503	PLRP-S	5 µm	20 mm	€ 104.-	€ 442.-
TDR218503	Perfusion C18	10 µm 5 µm	30 mm 10 mm	€ 137.-	€ 583.-
TT18SC183	C18 SCX C18	5 µm 5 µm 5 µm	15 mm 10 mm 20 mm	€ 162.-	€ 689.-
TT18PP183	C18 TiO₂ C18	5 µm 5 µm 5 µm	15 mm 3 mm 20 mm	€ 162.-	€ 689.-

[Top]

100-µm ID Trapping Columns

Recommended for use with our 50-µm and 75-µm ID Analytical Columns
Optimal mobile phase flow rate at trapping stage: 3-5 µL/min

Catalog Number	Column Chemistry	Particle Size	Bed Length	Price (1/pk)	Price (5/pk)
TS04504	C4	5 µm	20 mm	€ 93.-	€ 396.-
TS08504	C8	5 µm	20 mm	€ 93.-	€ 396.-
TS18504	C18	5 µm	20 mm	€ 93.-	€ 396.-
TSPR504	PLRP-S	5 µm	20 mm	€ 104.-	€ 442.-
TDR218504	Perfusion C18	10 µm 5 µm	30 mm 10 mm	€ 137.-	€ 583.-
TT18SC184	C18 SCX C18	5 µm 5 µm 5 µm	15 mm 10 mm 20 mm	€ 162.-	€ 689.-
TT18PP184	C18 TiO₂ C18	5 µm 5 µm 5 µm	15 mm 3 mm 20 mm	€ 162.-	€ 689.-

[Top]

200-µm ID Trapping Columns

Recommended for use with our 100-µm or 200-µm ID Analytical Columns
The Porous Graphitic Carbon (PGC) Column is recommended for offline fractionation in combination with our Strong Cation eXchange Analytical Columns
Optimal mobile phase flow rate at trapping stage: 5-10 µL/min

Catalog Number	Column Chemistry	Particle Size	Bed Length	Price (1/pk)	Price (5/pk)
TS04508	C4	5 µm	20 mm	€ 93.-	€ 396.-
TS08508	C8	5 µm	20 mm	€ 93.-	€ 396.-
TS18508	C18	5 µm	20 mm	€ 93.-	€ 396.-
TSGC708	PGC	7 µm	5 mm	€ 104.-	€ 442.-
TSPR508	PLRP-S	5 µm	20 mm	€ 104.-	€ 442.-
TDR218508	Perfusion C18	10 µm 5 µm	30 mm 10 mm	€ 137.-	€ 583.-
TT18SC188	C18 SCX C18	5 µm 5 µm 5 µm	15 mm 10 mm 20 mm	€ 162.-	€ 689.-
TT18PP188	C18 TiO₂ C18	5 µm 5 µm 5 µm	15 mm 3 mm 20 mm	€ 162.-	€ 689.-

[Top]

* The First Order Discount (15%) is available for new customers placing their first order. To ensure fair use, the offer is limited to one use per company. We may verify eligibility using the company’s VAT number, business registration, or other identifying details. Multiple orders from the same company may not qualify. Please note that the discount applies only to items classified as ‘Goods’ as defined in our General Terms and Conditions.

VAT Number:	NL003034706B09
Chamber of Commerce:	97696285